![]() ![]() Our findings show that, when compared to conventional out-of-cage phenotyping, a far deeper understanding of mouse mutant phenotype can be established by monitoring behaviour in the home cage over one or more light:dark cycles.īroad based phenotyping of genetically altered mice employs a battery of tests that, when taken together, provide very useful insights into the influence of the target gene on behaviour ( Crawley and Paylor, 1997, Loos et al., 2014). Secondly, we tested a number of relevant mutant lines to determine how discriminative these parameters were. Firstly, using these systems, we explored behaviours in a number of mouse inbred strains to determine whether we could extract biologically meaningful differences. In teasing out the elements of this test that have determined its robustness – automated assessment of an unforced behaviour in the home cage over long time intervals – we and others have been investigating whether similar test apparatus could be used to accurately discriminate differences in distinct behavioural parameters in mice. Furthermore, this assay has been instrumental in dissecting the molecular genetic basis of mammalian circadian rhythms. Historically, wheel-running in mice and other rodents have been used as a robust assay to determine, with precision, the inherent period of circadian rhythms in mice. Our experience has shown that this has been most evidenced in long-term assessment of wheel-running activity in mice. An important factor in reducing variability in mouse test outcomes has been to develop assays that can be used for continuous automated home cage assessment. ![]()
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